The protective effect of 20% ethanol-treated oocysts of Eimeria species against coccidiosis in layer chickens was investigated in the present study. Local eimerian oocysts were treated with 20% ethanol during and after sporulation for 48 h. A total of 180 male layer chicks were randomly divided into five groups: group 1 (G1, a negative control group without treatment or infection); group 2 (G2, a positive control group) received untreated sporulated oocysts at 21 days old; group 3 (G3) was inoculated orally with 1x104 non -treated sporulated oocysts at 4 days old; group 4 (G4) and group 5 (G5) were administered 20% ethanol-treated unsporulated oocysts (sporulate during treatment) (1x104) or 20% ethanol- treated-sporulated oocysts (1x104). All groups except G1 were challenged at day 21 of age with 7.5×104 untreated sporulated oocysts as a single dose. At day 7 post-immunization, chicks in G4 and G5 showed less severity of bloody diarrhea in a significant manner and a decreased number of oocysts were shed, which were not able to sporulate. Post-challenge results showed that G4 and G5 birds with mild bloody diarrhea and cecal lesions, with a lower number of oocysts, and better body weight gain than in G2 birds. Moreover, birds which were inoculated with ethanol-treated oocysts had higher levels of cytokines post-challenge than controls (G2 and G3). In conclusion, the treatment of oocysts with 20% ethanol provided a certain level of protection against Eimeria infection.
OBJECTIVE: To measure and compare the expression of miRNA 210-5p, miR-195-5p, Vascular endothelial growth factor (VEGF), Vascular endothelial growth factor receptor-1 (VEGFR-1), Vascular endothelial growth factor receptor-2 (VEGFR-2) and soluble Flt-1 (sFlt-1) in the preeclamptic and normotensive pregnant women. To check the correlation of miRNA-210-5p and miR-195-5p with mRNA expression of VEGF and its receptors in preeclampsia. METHODS: This cross sectional analytical study was conducted in the department of cell biology and physiology, University of Health Sciences, Lahore from November 2017 till December 2018. Diagnosed cases of preeclampsia with age matched normotensive women were the study participants. All the participants were in third trimester of pregnancy. Blood samples were collected and real-time quantitative reverse transcription polymerase chain reaction was performed to analyze the expression of miRNA 210-5p, miR-195-5p, Vascular endothelial growth factor receptor (VEGF), Vascular endothelial growth factor receptor-1 (VEGFR-1), Vascular endothelial growth factor receptor-2 (VEGFR-2) and soluble Flt-1 (sFlt-1). Threshold cycle values were recorded and used to calculate fold change using 2-??ct method. SPSS version 22 was used to analyze the data. RESULTS: The expression level of miRNA 210-5p and miR-195-5p was increased in the preeclamptic group by a 2.97 and 1.57 fold respectively. Upregulated miR-210-5p and 195-5p showed an inverse correlation with VEGF, VEGFR-1 and VEGFR2 and a strong positive correlation with sFlt-1 (p value<0.001). CONCLUSION: The study concludes that upregulated miRNA-195-5p and miR-210-5p along with differentially expressed angiogenic VEGF and its receptors might play an important role in deregulation of angiogenesis involved in the pathogenesis of preeclampsia.
Geothermal energy sources are widely used for heating as well as for cooling applications. Low energy houses are prefered for this kind of energy source because of its benefits. In this work it has been investigated a groud source heat pump (GSHP) that is connected to ventilo-convector units (VCUs) for active heating. For cooling it has been investigated passive cooling that presents a low energy consumption close to zero, only the pumping energy is necessary. The initial building has no insulation and gas boiler with low efficiency for DHW and heating purposses. For all the simulations Designbuilder software has been used, that presents detailed energy consumption for all types of consumers from the studied building: heating, cooling, lighting, and domestic hot water production. The type and caracteristics of soil is introduced from other measured boreholes, from Bucharest, with Thermal Response Test (TRT) method.
Abstract\nBackground & Objective: Emotional Intelligence promotes organizational creativity, improves the ability to communicate with yourself and others, and ultimately enhances job performance. This paper is intended to evaluate the EI level among personnel based on the hospitals’ type of ownership. Materials & Methods: A descriptive, analytical study was performed within a cross-sectional setting in 2019 on 2850 personnel of five selected hospitals in Mazandaran province (Iran) with different ownerships. Standard Shearing\'s EI 33-item questionnaire was used. Six hundred questionnaires were distributed among participants. Data were analyzed in SPSS 22 using One Way ANOVA (Analysis of Variance) with post-hoc Tukey’s HSD test (P < 0.05). \nResults: Five hundred sixty-nine questionnaires were completed with a mean response rate of 94.8%. Mean EI scores among personnel of therapeutic, private, educational, and social security hospitals were 110.74 ± 14.72, 119.09 ± 16.40, 112.06 ± 11.49 and 117.40 ± 13.90, respectively, indicating a significant relationship among hospitals (P=0.001). The highest mean EI scores were observed among personnel of social security hospital in terms of self-motivation (24.3 ± 92.67), consciousness (30.4 ± 51.53), social consciousness (21.3 ± 04.26), social skills (17.3 ± 44.02), and self-mastery (23.3 ± 49.56). Conclusion: The EI level among social security hospital personnel was higher than that of educational and therapeutic centers, and therapeutic and private hospitals.\nKeywords: Hospitals, Emotional Intelligence, Ownership