Abiotic stress is the main source of danger for many crop plants. Salinity and droughts are the most common categories of this stress all over the world. In this study, Transformation of EsWRKY11 in Oryza sativa (Giza 177) enhanced salt tolerance at the seedling stage. As Oryza satvia plant is considered one of the most important crops, it ranks the most second important cereal in the world. Oryza satvia production represents 30% of the world’s cereal production today. After many searches, it was found that the WRKY transcription factors play an important role in the regulation of transcriptional reprogramming associated with plant abiotic stress responses. In this study, the WRKY transcription factor EsWRKY11, containing the plant-specific WRKY zinc finger DNA-binding motif, was isolated from Eruca sativa. The source of the WRKY11 gene is members of family Brassicaceae such as Arabidopsis thaliana and Eruca sativa. Nanoparticles are a new trend technique applied for gene transformation. For example, chitosan nanoparticles are biocompatible, biodegradable, and give high transformation efficiency. The transformed EsWRKY11 gene was detected in rice transgenic lines. EsWRKY11 was upregulated in response to a variety of abiotic stresses, including salinity, alkalinity, cold, abscisic acid, and drought. Micropropagation tissue culture technique was used for the multiplication of both transgenic and non-transgenic rice lines. Transgenic Oryza sativa L. had a better salt-tolerant phenotype, and the hypocotyls were significantly longer than those of non-transgenic seeds after salt and drought treatment.