The study aims to find out the first genuses of lacustrine (non-marine) diatoms from the Miocene period of western Anatolia, Turkey. Therefore, samples are collected from three different diatomite strata in the ooze unit (diatomite and chalk togetherness) in the sedimentary sequence in the Seydiler region (Afyonkarahisar, Turkey), and the lacustrine diatom faunas of the samples were determined using FEI Nova Nano 650 Scanning Electron Microscope with Energy Dispersive X-Ray Spectrometer (SEM-EDX).This study area covers the Seydiler region, which is one of the deposit areas in one of the lakes that remained from Paleo-Tethys in the Miocene period of the Western Anatolia. In the study, Ellerbeckia, Eunotia, Gomphonema, Halamphora, Pinnularia, and Staurosira were found to be the major diatom faunas in the Seydiler ooze deposit. All three genuses of the Staurosira genus namely, Staurosira construens, Staurosirella pinnata3, and Staurosirella pinnata6, were entirely imaged and named in this floristic result.As can be seen, these preliminary genuses form the benthic freshwater diatom fauna, which symbolizes the shallow paleo-lacustrine environment and cold climate conditions.