This paper investigates collaboration among actors in the West Java horticultural agricultural entrepreneurship ecosystem. The descriptive-qualitative research method was used in this paper, emphasizing the quadruple helix between farming actors, government, universities, and the agricultural industry, analyzing the knowledge transfer process carried out among the actors involved in developing rural entrepreneurship. According to the study, the essential aspect of creating a competitive and productive agricultural entrepreneurship ecosystem is strengthening human resources and agricultural institutions. These programs have aided farmers in developing their farms on-farm and farm management. However, program implementation in the field remains limited and unsustainable. To overcome this, the involved actors must work together synergistically (government, universities, industry, and farming). Intense openness of information and communication can aid the synergy process, allowing the actors involved to strengthen each other and achieve the desired outcomes, creating a conducive, productive, and competitive agricultural entrepreneurship ecosystem.